Phenotyping the kinematics of leaf development in flowering plants: recommendations and pitfalls
Maryline Lièvre
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
These authors equally contributed to the article.Search for more papers by this authorNathalie Wuyts
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
VIB Department of Plant Systems Biology, Ghent University, Gent, Belgium
These authors equally contributed to the article.Search for more papers by this authorSarah J. Cookson
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Ecophysiologie et Génomique Fonctionnelle de la Vigne, INRA, UMR 1287, Villenave d'Ornon, France
These authors equally contributed to the article.Search for more papers by this authorJustine Bresson
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Laboratory of Tropical and Mediterranean Symbioses (UMR113), Université Montpellier 2, IRD, Cirad Supagro, INRA, Université Montpellier 2, CC002, Place E. Bataillon, Montpellier, France
These authors equally contributed to the article.Search for more papers by this authorMélanie Dapp
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Laboratory of Plant Genetics Sciences III, Geneva 4, Switzerland
These authors equally contributed to the article.Search for more papers by this authorFrançois Vasseur
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCatherine Massonnet
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Ecologie et Ecophysiologie Forestières, UMR 1137 INRA/UL, Champenoux, France
Search for more papers by this authorSébastien Tisné
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorMathilde Bettembourg
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCrispulo Balsera
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorAlexis Bédiée
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorFrédéric Bouvery
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorMyriam Dauzat
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorGaëlle Rolland
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorDenis Vile
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCorresponding Author
Christine Granier
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Correspondence to: [email protected]Search for more papers by this authorMaryline Lièvre
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
These authors equally contributed to the article.Search for more papers by this authorNathalie Wuyts
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
VIB Department of Plant Systems Biology, Ghent University, Gent, Belgium
These authors equally contributed to the article.Search for more papers by this authorSarah J. Cookson
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Ecophysiologie et Génomique Fonctionnelle de la Vigne, INRA, UMR 1287, Villenave d'Ornon, France
These authors equally contributed to the article.Search for more papers by this authorJustine Bresson
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Laboratory of Tropical and Mediterranean Symbioses (UMR113), Université Montpellier 2, IRD, Cirad Supagro, INRA, Université Montpellier 2, CC002, Place E. Bataillon, Montpellier, France
These authors equally contributed to the article.Search for more papers by this authorMélanie Dapp
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Laboratory of Plant Genetics Sciences III, Geneva 4, Switzerland
These authors equally contributed to the article.Search for more papers by this authorFrançois Vasseur
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCatherine Massonnet
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Ecologie et Ecophysiologie Forestières, UMR 1137 INRA/UL, Champenoux, France
Search for more papers by this authorSébastien Tisné
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorMathilde Bettembourg
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCrispulo Balsera
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorAlexis Bédiée
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorFrédéric Bouvery
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorMyriam Dauzat
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorGaëlle Rolland
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorDenis Vile
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Search for more papers by this authorCorresponding Author
Christine Granier
Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, Montpellier, France
Correspondence to: [email protected]Search for more papers by this authorConflict of interest: The authors have declared no conflicts of interest for this article.
Abstract
Leaves of flowering plants are produced from the shoot apical meristem at regular intervals and they grow according to a developmental program that is determined by both genetic and environmental factors. Detailed frameworks for multiscale dynamic analyses of leaf growth have been developed in order to identify and interpret phenotypic differences caused by either genetic or environmental variations. They revealed that leaf growth dynamics are non-linearly and nonhomogeneously distributed over the lamina, in the leaf tissues and cells. The analysis of the variability in leaf growth, and its underlying processes, has recently gained momentum with the development of automated phenotyping platforms that use various technologies to record growth at different scales and at high throughput. These modern tools are likely to accelerate the characterization of gene function and the processes that underlie the control of shoot development. Combined with powerful statistical analyses, trends have emerged that may have been overlooked in low throughput analyses. However, in many examples, the increase in throughput allowed by automated platforms has led to a decrease in the spatial and/or temporal resolution of growth analyses. Concrete examples presented here indicate that simplification of the dynamic leaf system, without consideration of its spatial and temporal context, can lead to important misinterpretations of the growth phenotype. WIREs Dev Biol 2013, 2:809–821. doi: 10.1002/wdev.119
This article is categorized under:
- Plant Development > Vegetative Development
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